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color dsfi1 camera  (Nikon)


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  • 90

    Structured Review

    Nikon color dsfi1 camera
    Color Dsfi1 Camera, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/color+dsfi1+camera/pmc11705399-78-46-50
    Average 90 stars, based on 1 article reviews
    color dsfi1 camera - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Microscopy:

    Article Title: Fluorescent identification of axons, dendrites and soma of neuronal retinal ganglion cells with a genetic marker as a tool for facilitating the study of neurodegeneration
    Article Snippet: The cells were gently triturated and plated on a 12 mm Nunc glass base dish (Cat# 150680; Thermo Scientific) coated with poly‐D‐lysine and mouse laminin I. RGC culture media was adapted from published reports ; it contained a mix of DMEM (Cat# 11960044; Gibco) and neurobasal media (Cat# 21103049; Gibco), with insulin (5 μg/mL; Cat# I6634; Sigma), sodium pyruvate (1 mM; Cat# 11360–070; Sigma), NS21 supplement (Cat# AR008; R&D systems), penicillin/streptomycin (1%, Cat # 15140–12; Gibco), SATO supplement (1X; made in house containing Neurobasal Medium (Invitrogen), bovine serum albumin (100 μg/mL; Sigma, A4161), sodium selenite (40 ng/mL), putrescine (16 μg/mL), triiodo‐thyronine (1.6 μg/mL), apo‐transferrin (100 μg/mL; Sigma, T‐1147), and progesterone (60 ng/mL) as described), L‐glutamine (2 mM, Cat# 25030–081; Gibco), triiodothyronine (T3, 40 ng/mL, Cat# T6397; Sigma), brain‐derived neurotrophic factor (BDNF; 50 ng/mL; Cat# 450–02; Peprotech, Rocky Hill, NJ), ciliary neurotrophic factor (CNTF; 10 ng/mL; Cat# 450–13; Peprotech), and forskolin (4.2 ng/mL; Cat# F6886; Sigma Aldrich). .. RGCs continued to grow for at least 4 weeks, when monitoring stopped; neurite growth and soma numbers were monitored daily from live cultures with an inverted Nikon Eclipse tissue culture microscope with a TRITC filter set (544 nm excitation/570 nm emission), and images captured with a color DSFi1 camera (all Nikon Instruments). .. Levels of intracellular Ca 2+ in RGCs were determined microscopically based on approaches described previously., In brief, mixed retinal cultures 3–5 days after plating were loaded with 5 μM Fura‐2 AM (Thermo Fisher) with 0.01% pluronic F‐127 at 37°C for 45 min.



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